Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00546183" target="_blank" >RIV/61388955:_____/21:00546183 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10442137
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Construction and application of the amperometric uric acid biosensors based on the covalent immobilization of uricase by different strategies
Original language description
In this work, a promising combination of a biosensor based on thenenzymatic mini-reactor with the detection principle of four-electronnreduction of the consumed oxygen at highly negative potential hasnbeen developed for uric acid determination using flow injectionnanalysis. The construction of the biosensor provides a spatialnsegregation of the biorecognition (uricase-based mini-reactor) andndetection (tubular detector of silver solid amalgam (TD-p-AgSA))nparts. To find out the most appropriate enzyme immobilizationnprotocol, three different strategies of the covalent attachment fornuricase from Bacillus fastidiosus have been compared. It was foundnthat the biosensor with the mini-reactor based on the covalentnattachment of uricase via glutaraldehyde to -NH2 functionalizednmesoporous silica powder MCM-41 showed extremely high stabilityn(>1 year) and reusability (at least 600 measurements) The biosensor'snpractical applicability was confirmed by successful determinationnof uric acid in human urine.
Czech name
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Czech description
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Project
<a href="/en/project/GA20-01589S" target="_blank" >GA20-01589S: New strategies for improving sensing properties of novel electrode materials via their surface pretreatment or modification</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 17th ISC Modern Analytical Chemistry
ISBN
978-80-7444-089-2
ISSN
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e-ISSN
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Number of pages
7
Pages from-to
145-151
Publisher name
Charles University
Place of publication
Prague
Event location
Prague
Event date
Sep 16, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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