High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00568897" target="_blank" >RIV/61388955:_____/23:00568897 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10472886
Result on the web
<a href="https://hdl.handle.net/11104/0340175" target="_blank" >https://hdl.handle.net/11104/0340175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2023.142033" target="_blank" >10.1016/j.electacta.2023.142033</a>
Alternative languages
Result language
angličtina
Original language name
High-performance amperometric biosensor for flow injection analysis consisting of a replaceable lactate oxidase-based mini-reactor and a silver amalgam screen-printed electrode
Original language description
L-lactic acid (LA) is an essential compound in the winemaking process. Furthermore, it is considered a significant biomarker for the diagnosis of a wide range of diseases, including cancer. Therefore, a cost-effective and reliable biosensing system for FIA is developed here for rapid and selective determination of LA. The biosensor architecture includes an easily replaceable lactate oxidase (LOx)-based mini-reactor connected in front of a silver amalgam screen-printed electrode acting as a transducer. The mini-reactor represents a tube filled with mesoporous silica powder (SBA−15) coated with covalently immobilized LOx. The principle of LA determination is based on the amperometric monitoring of oxygen consumption by its four-electron reduction at −900 mV vs. Ag pseudo-reference electrode, which avoids the interferences from commonly interfering compounds. The application of a strategy of a spatially separated biorecognition and detection part allowed for the immobilization of a notably large amount of enzyme (one mini-reactor contains c.a. 270 μg of the LOx), which, in turn, ensured excellent operational (93.8% of the initial signal after 350 measurements) and storage (96.9% of the initial biosensor response after 7 months) stability. The newly developed high-performance biosensor was successfully tested for the quantification of LA in saliva, wine, and dairy products.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/GA20-07350S" target="_blank" >GA20-07350S: Electrochemical and analytical aspects of the transport of addictive and psychotropic drugs across the model biological barriers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica acta
ISSN
0013-4686
e-ISSN
1873-3859
Volume of the periodical
445
Issue of the periodical within the volume
MAR 2023
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
142033
UT code for WoS article
000990156800001
EID of the result in the Scopus database
2-s2.0-85148323718