An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10478221" target="_blank" >RIV/00216208:11310/23:10478221 - isvavai.cz</a>
Result on the web
<a href="https://www.bestservis.eu/realizovane-akce/42-mem/43/" target="_blank" >https://www.bestservis.eu/realizovane-akce/42-mem/43/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application
Original language description
An amperometric biosensor consisting of an enzymatic mini-reactor (lactate oxidase covalently attached to −NH2 functionalized mesoporous silica powder SBA−15 using glutaraldehyde) and a silver amalgam-based screen-printed electrode acting as a transducer was developed for the determination of L-lactic acid (LA) in FIA. The detection potential of −0.9 V vs. Ag pseudoreference electrode was applied for cathodic detection of enzymatically consumed oxygen. Under the optimized conditions, the constructed biosensor enabled selective determination of LA with a micromolar limit of detection. Importantly, the proposed biosensor represented excellent operational stability after ≥350 measurements. Finally, it was successfully applied to real sample analysis.
Czech name
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Czech description
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník přednášek mezinárodní odborné konference 42. Moderní Elektrochemické Metody
ISBN
978-80-908947-0-9
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
213-217
Publisher name
Lenka Srsenová - BEST Servis Ústí nad Labem
Place of publication
Ústí nad Labem
Event location
Jetrichovice, Česká republika
Event date
May 22, 2023
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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