Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33155875" target="_blank" >RIV/61989592:15310/15:33155875 - isvavai.cz</a>

Result on the web

<a href="http://journal.frontiersin.org/article/10.3389/fpls.2015.00862/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fpls.2015.00862/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.00862" target="_blank" >10.3389/fpls.2015.00862</a>

Result language

angličtina

Original language name

Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor

Original language description

Hydrogen peroxide (H2O2) is known to be generated in Photosystem II (PSII) via enzymatic and non-enzymatic pathways. Detection of H2O2 by different spectroscopic techniques has been explored; however, its sensitive detection has always been a challenge in photosynthetic research. During the recent past, fluorescence probes such as Amplex Red has been used but is known to either lack specificity or limitation with respect to the minimum detection limit of H2O2. We have employed an electrochemical biosensor for real time monitoring of H2O2 generation at the level of sub-cellular organelles. The electrochemical biosensor comprises of counter electrode and working electrodes. The counter electrode is a platinum plate while the working electrode is a mediator based catalytic amperometric biosensor device developed by the coating of a carbon electrode with osmium-horseradish peroxidase which acts as H2O2 detection sensor. In the current study, generation and kinetic behavior of H2O2 in PSII

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

862

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000364241800001

EID of the result in the Scopus database

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