An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152388" target="_blank" >RIV/00216305:26620/24:PU152388 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/24:00588275 RIV/00216224:14740/24:00138500 RIV/62156489:43210/24:43925374
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2024.111138" target="_blank" >10.1016/j.microc.2024.111138</a>
Alternative languages
Result language
angličtina
Original language name
An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples
Original language description
An enzyme-based electrochemical biosensor was fabricated for the sensitive determination of lactose. The utilized enzyme cascade system is composed of 8-galactosidase (8-Gal) and glucose oxidase (GOx). The ruthenium (IV) oxide (RuO2) presents in MWCNT-RuO2 nanocomposite immobilized on the glassy carbon electrode acts as an electrochemical mediator, resembling a second-generation enzyme biosensor. The functional mechanism of the biosensor was discussed, explaining the chemical oxidation of H2O2, the final product of the enzymatic reaction, by RuO2 and subsequent reoxidation of generated Ru to RuO2 at the electrode surface. This shifts the oxidation of H2O2 to a lower potential magnitude of +0.40 V, enhancing the biosensor's selectivity. The analytical figures of merit were verified for the developed lactose biosensor through repetitive measurements. The precision of the lactose biosensor was ensured with good reproducibility (RSD % = 2.68) and repeatability (RSD % = 4.12). The selectivity of the biosensor towards various saccharides and ionic species potentially present in milk samples was investigated, and no notable interference effect was detected. Moreover, the accuracy of the lactose biosensor was tested by analyzing spiked samples and a semi-skimmed milk (SS-milk) sample with certified lactose values. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be (0.036 mM) and (0.121 mM), respectively. Thanks to the short response time of the fabricated lactose biosensor, it was transferred to a screen-printed carbon electrode (SPCE) and successfully employed in flow injection analysis (FIA).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/EF18_046%2F0015974" target="_blank" >EF18_046/0015974: Upgrading Czech Infrastructure for Integrative Structural Biology</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
MICROCHEMICAL JOURNAL
ISSN
0026-265X
e-ISSN
1095-9149
Volume of the periodical
204
Issue of the periodical within the volume
111138
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
„“-„“
UT code for WoS article
001272028000001
EID of the result in the Scopus database
2-s2.0-85198242094