Determination of lactose in milk products: a comparison of three-enzyme amperometric biosensor and gas chromatography/tandem mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73583927" target="_blank" >RIV/61989592:15310/17:73583927 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00706-016-1903-7" target="_blank" >https://link.springer.com/article/10.1007%2Fs00706-016-1903-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-016-1903-7" target="_blank" >10.1007/s00706-016-1903-7</a>

Result language

angličtina

Original language name

Determination of lactose in milk products: a comparison of three-enzyme amperometric biosensor and gas chromatography/tandem mass spectrometry

Original language description

Two completely different analytical methods for lactose determination are presented. Three-enzyme biosensor involving b-galactosidase, glucose oxidase, and horseradish peroxidase was constructed with successive steps of optimization. Primary experiments were carried out with horseradish peroxidase biosensor in solution of hydrogen peroxide. Addition of poly(ethylene glycol) diglycidyl ether was examined and the best constant potential for chronoamperometric experiments was selected at -0.05 V using cyclic voltammetry. Ferrocene was chosen as the best from three tested mediators and optimum concentration of ferrocene was found out at 2 g dm(-3). Two electrode materials were compared. Spectroscopic graphite electrode proved better electrochemical response for its twice larger electro-active surface than screen-printed carbon electrode. Phosphate buffer solution of pH 7.5, at which the three-enzyme system provided the highest current signal, was used for lactose determination in milk, milk powder, curd cheese, and yogurt. Gas chromatography with tandem mass spectrometric detection was used for determination of lactose, glucose, and galactose in the real samples with internal standard salicine using single ion monitoring mode. Determined content of lactose in the milk products was compared with declared values. Gas chromatography with tandem mass spectrometric detection offers higher sensitivity and orthogonal selectivity in comparison to enzyme biosensor.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

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Volume of the periodical

148

Issue of the periodical within the volume

3

Country of publishing house

AT - AUSTRIA

Number of pages

8

Pages from-to

517-524

UT code for WoS article

000396528600017

EID of the result in the Scopus database

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