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Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00543208" target="_blank" >RIV/61388955:_____/21:00543208 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/21:10429959

  • Result on the web

    <a href="http://hdl.handle.net/11104/0320478" target="_blank" >http://hdl.handle.net/11104/0320478</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.snb.2021.130252" target="_blank" >10.1016/j.snb.2021.130252</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization

  • Original language description

    This is the first report of a novel strategy for uric acid (UA) determination via newly developed highly stable flow biosensors based on different enzymatic mini-reactors in combination with a four-electron reduction of the consumed oxygen. The design of the biosensors provides a spatial separation of the detection (working electrode) and biorecognition (uricase-based mini-reactor) part. To prepare the most efficient enzymatic mini-reactor, a comparative study focused on three different techniques of the covalent uricase immobilization at two kinds of mesoporous silica powders (SBA−15 and MCM−41) and the physical enzyme adsorption at four types of carbonaceous powders (glassy carbon, graphite, Starbon® 300, and Starbon® 800) has been performed. All investigations were carried out for two kinds of uricase: from Candida sp. (UOx(c)) and from Bacillus fastidiosus (UOx(b)) to estimate the influence of uricase source. The flow-through cell with tubular detector of silver solid amalgam was used for amperometric monitoring of four-electron oxygen reduction at the highly negative potential (−1100 mV vs. SCE). It was found that the developed UA biosensors responses are strongly dependent on the enzyme immobilization strategy. UOx(c) bounded to glassy carbon by physical adsorption has provided good results in terms of sensitivity, stability, and repeatability for UA determination. The biosensor with the mini-reactor based on the covalent immobilization of UOx(b) at glutaraldehyde activated single bondNH2 functionalized MCM–41 has shown extremely high stability (more than 1 year) and reusability (at least 600 measurements) The biosensor’s practical applicability has been tested by successful determination of UA in human urine.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10403 - Physical chemistry

Result continuities

  • Project

    <a href="/en/project/GA20-07350S" target="_blank" >GA20-07350S: Electrochemical and analytical aspects of the transport of addictive and psychotropic drugs across the model biological barriers</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Sensors and Actuators B - Chemical

  • ISSN

    0925-4005

  • e-ISSN

    0925-4005

  • Volume of the periodical

    344

  • Issue of the periodical within the volume

    OCT 2021

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    13

  • Pages from-to

    130252

  • UT code for WoS article

    000679299600005

  • EID of the result in the Scopus database

    2-s2.0-85107759684