Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00543208" target="_blank" >RIV/61388955:_____/21:00543208 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/21:10429959
Result on the web
<a href="http://hdl.handle.net/11104/0320478" target="_blank" >http://hdl.handle.net/11104/0320478</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2021.130252" target="_blank" >10.1016/j.snb.2021.130252</a>
Alternative languages
Result language
angličtina
Original language name
Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization
Original language description
This is the first report of a novel strategy for uric acid (UA) determination via newly developed highly stable flow biosensors based on different enzymatic mini-reactors in combination with a four-electron reduction of the consumed oxygen. The design of the biosensors provides a spatial separation of the detection (working electrode) and biorecognition (uricase-based mini-reactor) part. To prepare the most efficient enzymatic mini-reactor, a comparative study focused on three different techniques of the covalent uricase immobilization at two kinds of mesoporous silica powders (SBA−15 and MCM−41) and the physical enzyme adsorption at four types of carbonaceous powders (glassy carbon, graphite, Starbon® 300, and Starbon® 800) has been performed. All investigations were carried out for two kinds of uricase: from Candida sp. (UOx(c)) and from Bacillus fastidiosus (UOx(b)) to estimate the influence of uricase source. The flow-through cell with tubular detector of silver solid amalgam was used for amperometric monitoring of four-electron oxygen reduction at the highly negative potential (−1100 mV vs. SCE). It was found that the developed UA biosensors responses are strongly dependent on the enzyme immobilization strategy. UOx(c) bounded to glassy carbon by physical adsorption has provided good results in terms of sensitivity, stability, and repeatability for UA determination. The biosensor with the mini-reactor based on the covalent immobilization of UOx(b) at glutaraldehyde activated single bondNH2 functionalized MCM–41 has shown extremely high stability (more than 1 year) and reusability (at least 600 measurements) The biosensor’s practical applicability has been tested by successful determination of UA in human urine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
<a href="/en/project/GA20-07350S" target="_blank" >GA20-07350S: Electrochemical and analytical aspects of the transport of addictive and psychotropic drugs across the model biological barriers</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Sensors and Actuators B - Chemical
ISSN
0925-4005
e-ISSN
0925-4005
Volume of the periodical
344
Issue of the periodical within the volume
OCT 2021
Country of publishing house
CH - SWITZERLAND
Number of pages
13
Pages from-to
130252
UT code for WoS article
000679299600005
EID of the result in the Scopus database
2-s2.0-85107759684