Chiral separation of beta-blockers by high-performance liquid chromatography and determination of bisoprolol enantiomers in surface waters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10420690" target="_blank" >RIV/00216208:11310/20:10420690 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UKm9EIJ~b-" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UKm9EIJ~b-</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/aiht-2020-71-3318" target="_blank" >10.2478/aiht-2020-71-3318</a>
Alternative languages
Result language
angličtina
Original language name
Chiral separation of beta-blockers by high-performance liquid chromatography and determination of bisoprolol enantiomers in surface waters
Original language description
Beta-blockers are chiral compounds with enantiomers that have different bioactivity, which means that while one is active, the other can be inactive or even harmful. Due to their high consumption and incomplete degradation in waste water, they may reach surface waters and affect aquatic organisms. To address this issue we developed a chromatographic method suitable for determining beta-blocker enantiomers in surface waters. It was tested on five beta-blockers (acebutolol, atenolol, bisoprolol, labetalol and metoprolol) and validated on bisoprolol enantiomers. Good enantioseparation of all analysed beta-blockers was achieved on the Chirobiotic V column with the mobile phase composed of methanol/acetic acid/triethylamine (100/0.20/0.15 v/v/v) at a flow rate of 0.5 mL/min and colt= temperature of 45 degrees C. Method proved to be linear in the concentration range from 0.075 gginiL to 5 mu g/mL, and showed good recovery. The limits of bisoprolol enantiomer detection were 0.025 mu g/mL and 0.026 mu g/mL and of quantification 0.075 mu g/mL and 0.075 mu g/mL. Despite its limitations, it seems to be a promising method for bisoprolol enantiomer analysis in surface water samples. Further research could focus on waste water analysis, where enantiomer concentrations may be high. Furthermore, transferring the method to a more sensitive one such as liquid chromatography coupled with tandem mass spectrometry and using ammonium acetate as the mobile phase additive instead of acetic acid and triethylamine would perhaps yield much lower limits of detection and quantification.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Arhiv za Higijenu Rada i Toksikologiju
ISSN
0004-1254
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
1
Country of publishing house
HR - CROATIA
Number of pages
7
Pages from-to
56-62
UT code for WoS article
000525345900006
EID of the result in the Scopus database
2-s2.0-85087253647