Mixed-mode hydrophilic interaction/ion-exchange liquid chromatography ? Separation potential in peptide analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10435850" target="_blank" >RIV/00216208:11310/21:10435850 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FV_SqRq.UN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FV_SqRq.UN</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.microc.2021.106158" target="_blank" >10.1016/j.microc.2021.106158</a>
Alternative languages
Result language
angličtina
Original language name
Mixed-mode hydrophilic interaction/ion-exchange liquid chromatography ? Separation potential in peptide analysis
Original language description
In this work, three recently developed HILIC columns, i.e., HILIC-A containing silica gel, HILIC-N containing polyhydroxy phase and HILIC-B containing aminopropyl, were characterized in detail to compare their peptide retention, selectivity, and peak symmetry. The detailed study of chromatographic behavior of peptides revealed that a multimodal retention mechanism is present in systems with these stationary phases. Using three model analytes (caffeine, benzenesulfonic acid, and benzyltrimethylammonium chloride), we demonstrated that the HILIC-A column behaves as a HILIC/cation exchanger, and the HILIC-B column as a HILIC/anion exchanger depending on the mobile phase pH and buffer concentration. Furthermore, the pH (ammonium formate, pH 2.1 and 3.5; ammonium acetate pH 5.5) and buffer concentration (10?50 mM) of the mobile phase affect peptide retention and peak symmetry. Satisfactory retention and peak shape of peptides can be obtained by balancing ionic and hydrophilic properties of both analyte and stationary phases. Therefore, buffer concentration and pH optimization is an effective tool for manipulating retention and peak symmetry, thereby enhancing the potential of these columns in the analysis of diverse compounds. The potential application of these columns in the separation of biologically active peptides was confirmed by achieving baseline separation under optimized gradient elution.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microchemical Journal
ISSN
0026-265X
e-ISSN
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Volume of the periodical
165
Issue of the periodical within the volume
June
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
106158
UT code for WoS article
000647789500009
EID of the result in the Scopus database
2-s2.0-85103124991