Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008423" target="_blank" >RIV/00216275:25310/09:00008423 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants

Original language description

The retention of phenolic acid and flavone antioxidants was investigated on three columns with bonded PEG, hydroxy (DIOL) and mixed oxyethylene-hydroxy (Luna HILIC) stationary phases in buffered aqueous ACN mobile phases. All columns show mixed retentionmechanism: reversed phases in highly aqueous mobile phases and normal phases (HILIC, hydrophilic interaction LC) in mobile phases with high concentration of ACN, showing characteristic U-shape retention versus mobile phase composition plots. Equations enabling description and prediction of retention of sample compounds over the full mobile phase composition range were compared. Dual retention mechanism offers possibilities for using differences in selectivity between the two separation modes on a single column to improve the separation of natural antioxidants. The Luna HILIC column shows larger HILIC retention range, enhanced retention, improved selectivity and better peak symmetry in comparison to the PEG and DIOL columns.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: New analytical methods in combined high-capacity phase separation systems</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

32

Issue of the periodical within the volume

21

Country of publishing house

DE - GERMANY

Number of pages

17

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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