Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10445172" target="_blank" >RIV/00216208:11310/22:10445172 - isvavai.cz</a>

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S-FOybf46o" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S-FOybf46o</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2022.116095" target="_blank" >10.1016/j.jelechem.2022.116095</a>

Result language

angličtina

Original language name

Comparison of two pyrolytic graphite representatives in the construction of hybrid electrochemical DNA biosensors for monitoring DNA damage

Original language description

Electrochemical DNA biosensors represent a variety of versatile analytical tools successfully used in many important applications. Their utilization in the area of monitoring DNA damage is considered as the prominent field that can provide us with an improved general knowledge regarding DNA damaging events occurring in vivo by an unorthodox overall insight employing the various electrochemical methods. Herein, we present development, optimization process, and subsequent analytical testing of the novel hybrid electrochemical DNA biosensors based on the two representatives of the pyrolytic graphite - &quot;basal-plane&quot; pyrolytic graphite (BPPG) and &quot;edge-plane&quot; pyrolytic graphite (EPPG) - and low-molecular-weight double-stranded DNA (dsDNA). Closer resolution of the nature of the oxidation/reduction signals of the dsDNA, as well as the most important optimization of parameters securing satisfying operational stability of the proposed hybrid biosen-sors, is investigated. Subsequent analytical testing of the novel hybrid biosensors and the evaluation of their advantages/disadvantages in terms of monitoring DNA damage caused by an UV light irradiation employing various voltammetric methods (square wave (SWV), linear sweep (LSV), or cyclic (CV) voltammetry) and elec-trochemical impedance spectroscopy (EIS) is also presented.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GC20-01417J" target="_blank" >GC20-01417J: New approaches preventing passivation in monitoring of environmental organic pollutants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

1873-2569

Volume of the periodical

908

Issue of the periodical within the volume

March

Country of publishing house

CH - SWITZERLAND

Number of pages

9

Pages from-to

116095

UT code for WoS article

000767857500015

EID of the result in the Scopus database

2-s2.0-85124176855