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MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10451214" target="_blank" >RIV/00216208:11310/22:10451214 - isvavai.cz</a>

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.imlet.2022.10.007" target="_blank" >10.1016/j.imlet.2022.10.007</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology

  • Original language description

    Immunology is a rapidly evolving field of research with sophisticated models and methods. However, detailed data on total immune cell counts and population distributions remain surprisingly scarce. Nevertheless, recently established quantitative approaches could help us understand the overall complexity of the immune system. Here, we studied a major histocompatibility complexclass II - enhanced green fluorescent protein knock-in mouse model to precisely identify and manipulate lymphoid structures. By combining flow cytometry with light sheet microscopy, we quantified MHC II+ populations of the small intestine and associated individual mesenteric lymph nodes, with 36.7 x 10(6) cells in lamina propria, 3.0 x 10(5) cells in scattered lymphoid tissue and 1.1 x 10(6) cells in Peyer&apos;s patches. In addition to these whole-organ cell counts, we assessed approximately 1 x 10(6) total villi in the small intestine and 450 scattered lymphoid tissue follicles. By direct noninvasive microscopic observation of a naturally fully translucent mouse organ, the cornea, we quantified 12 +/- 4 and 35 +/- 7 cells/mm(2) Langerhans-and macrophage-like populations, respectively. Ultimately, our findings show that flow cytometry with quantitative imaging data analysis enables us to avoid methodological discrepancies while gaining new insights into the relevance of organ-specific quantitative approaches for immunology.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30102 - Immunology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Immunology Letters

  • ISSN

    0165-2478

  • e-ISSN

    1879-0542

  • Volume of the periodical

    251-252

  • Issue of the periodical within the volume

    12/2022

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    75-85

  • UT code for WoS article

    000886916300003

  • EID of the result in the Scopus database

    2-s2.0-85141831116