MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10451214" target="_blank" >RIV/00216208:11310/22:10451214 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.imlet.2022.10.007" target="_blank" >10.1016/j.imlet.2022.10.007</a>
Alternative languages
Result language
angličtina
Original language name
MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology
Original language description
Immunology is a rapidly evolving field of research with sophisticated models and methods. However, detailed data on total immune cell counts and population distributions remain surprisingly scarce. Nevertheless, recently established quantitative approaches could help us understand the overall complexity of the immune system. Here, we studied a major histocompatibility complexclass II - enhanced green fluorescent protein knock-in mouse model to precisely identify and manipulate lymphoid structures. By combining flow cytometry with light sheet microscopy, we quantified MHC II+ populations of the small intestine and associated individual mesenteric lymph nodes, with 36.7 x 10(6) cells in lamina propria, 3.0 x 10(5) cells in scattered lymphoid tissue and 1.1 x 10(6) cells in Peyer's patches. In addition to these whole-organ cell counts, we assessed approximately 1 x 10(6) total villi in the small intestine and 450 scattered lymphoid tissue follicles. By direct noninvasive microscopic observation of a naturally fully translucent mouse organ, the cornea, we quantified 12 +/- 4 and 35 +/- 7 cells/mm(2) Langerhans-and macrophage-like populations, respectively. Ultimately, our findings show that flow cytometry with quantitative imaging data analysis enables us to avoid methodological discrepancies while gaining new insights into the relevance of organ-specific quantitative approaches for immunology.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Immunology Letters
ISSN
0165-2478
e-ISSN
1879-0542
Volume of the periodical
251-252
Issue of the periodical within the volume
12/2022
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
75-85
UT code for WoS article
000886916300003
EID of the result in the Scopus database
2-s2.0-85141831116