Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10452864" target="_blank" >RIV/00216208:11310/22:10452864 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q13J3.LJ75" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=q13J3.LJ75</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-022-02924-w" target="_blank" >10.1007/s00706-022-02924-w</a>
Alternative languages
Result language
angličtina
Original language name
Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection
Original language description
Gradient elution for the determination of six selected neurotransmitters (3,4-dihydroxyphenylacetic acid, dopamine, homovanillic acid, 5-hydroxyindole-3-acetic acid, L-noradrenaline, serotonin) by HPLC with fluorescence detection on the C18 reverse phase was designed and optimized. The elution used the binary mobile phase of aqueous solution of 12x10(-3) mol dm(-3) acetic acid, 0.26x10(-3) mol dm(-3) Na2EDTA, pH = 3.5) and methanol. Furthermore, modified homogenization medium, prepared by mixing methanol and an aqueous solution (0.4 mol dm(-3) perchloric acid, 6x10(-3) mol dm-3 cysteine, 1x10(-3) mol dm(-3) L-ascorbic acid, and 0.54x10(-3) mol dm(-3) Na2EDTA) in a ratio of 1:1 (v/v), was introduced in the preparation of rat brain tissue to increase the stability of neurotransmitters in the sample before the analysis. Both developed procedures were successfully applied to the determination of selected neurotransmitters in authentic brain tissue samples from rats affected by morphine and/or morphine abstinence. The statistically significant increases in dopamine, 3,4-dihydroxyphenylacetic acid, and serotonin levels in the striatum, as well as L-noradrenaline in the cerebral cortex of rats affected by intraperitoneal administration of morphine, were measured.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA19-03295S" target="_blank" >GA19-03295S: Consequences of sustained morphine treatment and withdrawal on the rat brain: proteomic and functional studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Monatshefte für Chemie - Chemical Monthly
ISSN
0026-9247
e-ISSN
1434-4475
Volume of the periodical
153
Issue of the periodical within the volume
9
Country of publishing house
AT - AUSTRIA
Number of pages
6
Pages from-to
753-758
UT code for WoS article
000796806900002
EID of the result in the Scopus database
2-s2.0-85130479163