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LC-ESI-MS-MS Method for Monitoring Dopamine, Serotonin and Their Metabolites in Brain Tissue

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F11%3A43892006" target="_blank" >RIV/60461373:22310/11:43892006 - isvavai.cz</a>

  • Alternative codes found

    RIV/67985823:_____/11:00364479 RIV/00023752:_____/11:00001181 RIV/00216208:11120/11:00003214

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s10337-011-1959-9" target="_blank" >http://dx.doi.org/10.1007/s10337-011-1959-9</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10337-011-1959-9" target="_blank" >10.1007/s10337-011-1959-9</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    LC-ESI-MS-MS Method for Monitoring Dopamine, Serotonin and Their Metabolites in Brain Tissue

  • Original language description

    A rapid and precise LC-ESI-MS-MS method for the parallel identification and quantification of dopamine, serotonin and their metabolites (homovanillic acid, 3-methoxytyramine, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolacetic acid) from rat brain tissue without any pre-analysis adjustment of the sample such as pre-concentration or derivatization has been developed. In particular, the reaction-monitoring mode was selected for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. Alternation the ionization polarity in the course of mass spectrometry detection enabled to determine substances susceptible to various ionization modes in only one analysis run. This fact, in combination with an easy pre-treatment step, constitutes the method straightforward and time undemanding. The developed method was characterized with a high precision (?19.5%, determined as RSD), an acceptable accuracy (?82.0%, determined as recovery),

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chromatographia

  • ISSN

    0009-5893

  • e-ISSN

  • Volume of the periodical

    73

  • Issue of the periodical within the volume

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    7

  • Pages from-to

    143-149

  • UT code for WoS article

    000290804900017

  • EID of the result in the Scopus database