Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189883" target="_blank" >RIV/00216208:11110/13:10189883 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/13:00425848 RIV/60461373:22310/13:43896722 RIV/00179906:_____/13:10189883
Result on the web
<a href="http://dx.doi.org/10.1016/j.jpba.2013.03.026" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.03.026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2013.03.026" target="_blank" >10.1016/j.jpba.2013.03.026</a>

Result language
angličtina
Original language name
Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes
Original language description
A sensitive and precise method for simultaneous quantification of cysteinyl leukotrienes (=cys LTs) - leukotriene C-4 (=LTC4), leukotriene D-4 (=LTD4) and leukotriene E-4 (=LTE4) - essential biomarkers of bronchial asthma present in exhaled breath condensate (=EBC) was developed. An immunomagnetic molecular probe was prepared by anchoring cysteinyl leukotrienes antibody on the surface of functionalized monodispersed magnetic particles and used to selectively isolate cys LTs from biological matrices - EBC, plasma and urine. Immobilization and the immunoaffinity capture procedures were optimized to maximize the amount of separated cys LTs, which were detected "off-beads" after acidic elution by UHPLC-ESI-MS/MS operated in a multiple reaction monitoring mode. The developed method was characterized with high precision {= 13.6% (intra-day precision determined as RSD) and {= 14.5% (inter-day precision determined as RSD), acceptable accuracy {= 18.5% (determined as RE), and high recovery of i
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FC - Pneumology
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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