Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F11%3A9595" target="_blank" >RIV/00064165:_____/11:9595 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22310/11:43891926 RIV/60461373:22330/11:43891926 RIV/60461373:22330/11:43892670 RIV/60461373:22310/11:43892670 and 2 more
Result on the web
<a href="http://dx.doi.org/10.1016/j.jchromb.2011.06.004" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2011.06.004</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of cysteinyl leukotrienes in exhaled breath condensate: Method combining immunoseparation with LC-ESI-MS/MS
Original language description
A rapid and precise method for the identification and quantification of cysteinyl leukotrienes (leukotriene C(4), leukotriene D(4) and leukotriene E(4)), essential markers of bronchial asthma, in exhaled breath condensate was developed. The protocol consists of immunoaffinity separation and a detection step, liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized with a high precision (<= 7.7%, determined as RSD), an acceptable accuracy (90.4-93.7%, determined as recovery), a low limitof detection (<= 2 pg/ml EBC) and a low limit of quantification (<= 10 pg/ml EBC). It was compared to other simple, clinically appropriate combinations of pre-treatment methods (solid phase extraction and lyophilization) with LC/MS. Final
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FE - Other fields of internal medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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