Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography-tandem mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F11%3A43892008" target="_blank" >RIV/60461373:22310/11:43892008 - isvavai.cz</a>
Alternative codes found
RIV/00023752:_____/11:00001182 RIV/00216208:11120/11:00003215
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2011.02.006" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.02.006</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2011.02.006" target="_blank" >10.1016/j.chroma.2011.02.006</a>

Result language
angličtina
Original language name
Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography-tandem mass spectrometry
Original language description
A sensitive assay method was developed for a parallel, rapid and precise detn. of dopamine and its metabolites, homovanillic acid, 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid, from brain microdialyzates. The method consisted of a pretreatment step, freeze-drying (lyophilization), to conc. dopamine and its metabolites from the microdialyzates, and a detection step using liq. chromatog. combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the reaction monitoring mode was selected for its extremely high degree of selectivity and the stable-isotope-diln. assay for its high precision of quantification. The developed method was characterized by the following parameters: the precision of the developed method was detd. as ?88.6% for dopamine, ?89.9% for homovanillic acid, ?86.1% for 3-methoxytyramine and ?88.1% for 3,4-dihydroxyphenylacetic acid; the mean accuracy was detd. as ?88.2% for dopamine, ?88.3% for homovanillic acid, ?85.9% for 3-meth
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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