Simultaneous detection and quantification of two European anglerfishes by novel genomic primer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10453733" target="_blank" >RIV/00216208:11310/23:10453733 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/22:43925110 RIV/00027022:_____/22:N0000070

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vtqhXXw8PO" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=vtqhXXw8PO</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2022.104992" target="_blank" >10.1016/j.jfca.2022.104992</a>

Result language

angličtina

Original language name

Simultaneous detection and quantification of two European anglerfishes by novel genomic primer

Original language description

Globalisation has led to increased trade and consumption of fish worldwide. As international trade and con-sumption of fish increases, so does the likelihood of fish being adulterated. It is often an illegal exchange of species where a cheaper species is substituted for a more expensive and rarer one. In Europe, the anglerfish (Lophius piscatorius) is often traded as the rarer, more popular black-bellied anglerfish (Lophius budegassa). To improve our ability to monitor and detect adulterants in these fish species, we developed a real-time PCR assay that allows for the simultaneous detection and quantification of L. piscatorius and L. budegassa. The newly designed primers target the second intron of the genomic gene parvalbumin. The proposed methodology shows good robustness, efficiency and high specificity. Of the 47 species tested, only Lophius species were amplified. Their differentiation is possible by analysing melting curves with an average Tm of 70.1 °C for L. piscatorius and 75.3 °C for L. budegassa, respectively. The detection limit for both species was 0.050 ng/μl. The assay also provides a tool to quantify parvalbumin in European anglerfish. The findings point to the use of the method proposed as a potential tool in forensic investigations to prevent illegal species substitutions, mislabeling, and food fraud.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10613 - Zoology

Project

<a href="/en/project/QK1910231" target="_blank" >QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

1096-0481

Volume of the periodical

115

Issue of the periodical within the volume

January

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

104992

UT code for WoS article

000882031000006

EID of the result in the Scopus database

2-s2.0-85145862660