Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10466199" target="_blank" >RIV/00216208:11310/23:10466199 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap
Original language description
Vaccinia virus (VACV) is a prototypical poxvirus originally used for eradication of smallpox. Investigation into VACV mRNAs carried out almost half a century agosubstantially contributed to the fundamental discovery of the 5' mRNA cap, a hallmark of all eukaryotic and many viral mRNAs. VACV research also facilitated the identification and understanding of the general mechanism of 5' mRNA capsynthesis. We characterized the VACV transcripts at the individual mRNA molecule level and found that vaccinia postreplicative mRNAs, containing nontemplated 5' poly(A) leaders, surprisingly lack the 5' cap structure in vivo. We showing that 5' cap occurrence in viral mRNAs gradually decreases in eachsuccessive gene time classes, in contrast to the reciprocal increase in 5' poly(A)leader lengths, and that these two variables are mutually negatively correlated. Wealso demonstrate that the initiator region element (INR) directly or indirectlyinuences both the frequency of 5' mRNA capping and the occurrence of 5'poly(A) leaders, including their lengths in postreplicative VACV mRNAs.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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