Understanding the Effect of Aspartate Hydroxylase Inhibition On T Cell-Mediated Immunity Using Single-Cell RNA Sequencing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10467565" target="_blank" >RIV/00216208:11310/23:10467565 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Understanding the Effect of Aspartate Hydroxylase Inhibition On T Cell-Mediated Immunity Using Single-Cell RNA Sequencing
Original language description
Overexpression of aspartate β-hydroxylase (ASPH) was found in 70-90% of solid tumors but is negligible in normal tissues. Increased ASPH contributes to tumor cell proliferation, migration, and invasion but can also affect immune cells. In this study, we showed that ASPH inhibition with the small molecule inhibitor MO-I-1151 enhanced the anti-tumor effect stimulated by DNA immunization and activated an adaptive immunity mediated by CD4+ and CD8+ T cells. To reveal the mechanisms involved, we performed single-cell RNA sequencing with CD45+ tumor-infiltrating cells. Seurat and QIAGEN Ingenuity Pathway Analysis showed that inhibition of ASPH induced differences in infiltration with immune cells and cytokine/chemokine profiles of T cells in tumors of immunized mice. These results suggest a suppressive effect of ASPH on anti-tumor adaptive immunity that could be eliminated by ASPH inhibition.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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