Plasmid preparation: Optimization of 17-β-hydroxysteroid dehydrogenase 1 (HSD17B1) recombinant expression
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F23%3A10477837" target="_blank" >RIV/00216208:11310/23:10477837 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Plasmid preparation: Optimization of 17-β-hydroxysteroid dehydrogenase 1 (HSD17B1) recombinant expression
Original language description
Breast cancer, the most common cancer among women in western populations with a 10% lifetime risk, is largely driven by estrogen. Estradiol (E2), the most potent estrogen, promotes breast cancer growth. 17-β-hydroxysteroid dehydrogenases (HSD17B) catalyze E2 formation. HSD17B1, highly expressed in placenta, ovaries, and breast cancer tissue, is particularly prominent in aggressive hormone-dependent cancers, correlating with poor prognosis and short recurrence times. Inhibiting HSD17B1 presents a potential avenue for treating estrogen receptor-positive breast cancer.Attempts to express HSD17B1 in E. coli yielded minimal soluble protein and activity, limiting inhibitor screening via co-crystallization or enzyme assays. Expression in HEK293T cells resulted in low yields (approx. 2 mg/liter) of active protein from transient transfection. However, this expression level and purity are insufficient for cost-effective inhibitor screening. Additionally, wild-type enzyme activity diminishes upon long-term storage, necessitating the search for more stable protein mutants with higher yields, while preserving the active site and homodimer interface, as explored through the PROSS benchmark.
Czech name
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Czech description
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Classification
Type
V<sub>souhrn</sub> - Summary research report
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Number of pages
8
Place of publication
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Publisher/client name
IOCB TECH s.r.o., Flemingovo náměstí 542/2, 160 00 Praha 6, Česká republika
Version
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