Whole cell reconstructions of Leishmania mexicana through the cell cycle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10479647" target="_blank" >RIV/00216208:11310/24:10479647 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3JHTm2D8qK" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=3JHTm2D8qK</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.ppat.1012054" target="_blank" >10.1371/journal.ppat.1012054</a>
Alternative languages
Result language
angličtina
Original language name
Whole cell reconstructions of Leishmania mexicana through the cell cycle
Original language description
The unicellular parasite Leishmania has a precisely defined cell architecture that is inherited by each subsequent generation, requiring a highly coordinated pattern of duplication and segregation of organelles and cytoskeletal structures. A framework of nuclear division and morphological changes is known from light microscopy, yet this has limited resolution and the intrinsic organisation of organelles within the cell body and their manner of duplication and inheritance is unknown. Using volume electron microscopy approaches, we have produced three-dimensional reconstructions of different promastigote cell cycle stages to give a spatial and quantitative overview of organelle positioning, division and inheritance. The first morphological indications seen in our dataset that a new cell cycle had begun were the assembly of a new flagellum, the duplication of the contractile vacuole and the increase in volume of the nucleus and kinetoplast. We showed that the progression of the cytokinesis furrow created a specific pattern of membrane indentations, while our analysis of sub-pellicular microtubule organisation indicated that there is likely a preferred site of new microtubule insertion. The daughter cells retained these indentations in their cell body for a period post-abscission. By comparing cultured and sand fly derived promastigotes, we found an increase in the number and overall volume of lipid droplets in the promastigotes from the sand fly, reflecting a change in their metabolism to ensure transmissibility to the mammalian host. Our insights into the cell cycle mechanics of Leishmania will support future molecular cell biology analyses of these parasites. The parasite Leishmania causes the insect-transmitted neglected tropical disease, leishmaniasis. Leishmania is a single-celled parasite with a distinctive and highly defined shape. It is critical that each generation of the parasite retains the same shape. To achieve this, the parasite has a highly coordinated cell cycle in which organelles and key structures are duplicated and segregated at specific points. Previous work has defined a framework of cellular changes at the light microscopy level but this has limited resolution. We used volume electron microscopy to reconstruct Leishmania cells at different points in the cell cycle, which provided a three-dimensional overview of organelle positioning, duplication, and segregation. Moreover, we found that cytokinesis created membrane indentations that persisted in the daughter cells for a limited time. Finally, we examined Leishmania parasites in their insect vector, the sand fly. We found that the parasites in the sand fly had a greater number of lipid droplets, indicative of changes to their metabolism. Our insights into the cell cycle mechanics of Leishmania will provide a framework for future analyses of these parasites.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10600 - Biological sciences
Result continuities
Project
<a href="/en/project/GA21-15700S" target="_blank" >GA21-15700S: Leishmania-sand fly interaction: new approaches to answer old questions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Pathogens
ISSN
1553-7366
e-ISSN
1553-7374
Volume of the periodical
20
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
28
Pages from-to
e1012054
UT code for WoS article
001181692800002
EID of the result in the Scopus database
2-s2.0-85186751654