Historical analgesic tablets revisited: development of a versatile RP-HPLC method for the stability study of single- and multi-component pharmaceutical preparations
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F25%3A10504902" target="_blank" >RIV/00216208:11310/25:10504902 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Hskhs4dNcS" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Hskhs4dNcS</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-025-03350-4" target="_blank" >10.1007/s00706-025-03350-4</a>
Alternative languages
Result language
angličtina
Original language name
Historical analgesic tablets revisited: development of a versatile RP-HPLC method for the stability study of single- and multi-component pharmaceutical preparations
Original language description
A versatile RP-HPLC method using phenyl-hexyl stationary phase and gradient elution (0.1% aqueous acetic acid and methanol) was developed to investigate the chemical integrity of twelve historical analgesic single- and multi-component preparations (1940s to 1990s). UV-Vis detection, supported by tandem mass spectrometry, was employed for compound identification and potential degradation product screening. Twelve active pharmaceutical ingredients (APIs) - including acetylsalicylic acid, bromisoval, caffeine, codeine, narcotine, phenacetine, phenobarbital, salicylic acid, and four distinct barbiturates - were investigated. Calibration models were established with limits of quantification as low as 3.0 mg dm(-3). Coelution of phenobarbital and aminophenazone was effectively resolved through mathematical correction using dual-wavelength detection. Most APIs demonstrated remarkable long-term stability (within +/- 10% of declared content), with the exception of codeine and acetylsalicylic acid in specific preparation. Effervescent and poorly stored tablets exhibited significant degradation, highlighting the importance of dosage form and storage conditions. In addition, the tandem mass spectrum of bromisoval is presented here for the first time in the literature, and a plausible fragmentation mechanism is proposed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Monatshefte für Chemie - Chemical Monthly
ISSN
0026-9247
e-ISSN
1434-4475
Volume of the periodical
156
Issue of the periodical within the volume
10
Country of publishing house
AT - AUSTRIA
Number of pages
13
Pages from-to
1013-1025
UT code for WoS article
001543328600001
EID of the result in the Scopus database
2-s2.0-105012610330