Ion Channel Activity of Transmembrane Segment 6 of Escherichia coli Proton-Dependent Manganese Transporter

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F10%3A10062292" target="_blank" >RIV/00216208:11320/10:10062292 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Ion Channel Activity of Transmembrane Segment 6 of Escherichia coli Proton-Dependent Manganese Transporter

Original language description

Synthetic peptide corresponding to transmembrane segment TMS6 of secondary-active transporter MntH from Escherichia coli was used as a suitable alternative model enabling to study TMS structure, TMS interaction with membranes and also function of MntH. The secondary structure of the peptides was estimated in different environments using circular dichroism spectroscopy. These peptides interacted with and adopted helical conformation in lipid membranes. Electrophysiological experiments demonstrated that individual TMS were able under certain conditions to form ion channels in model biological membranes. Electrophysiological properties of these weakly cation-selective ion channels were strongly dependent on surrounding pH and the presence of manganese ion, as physiological substrate of MntH.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA204%2F07%2F0558" target="_blank" >GA204/07/0558: Biophysical study of MntH membrane transport proteins: structure-function relationship</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biopolymers

ISSN

0006-3525

e-ISSN

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Volume of the periodical

93

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000279035900005

EID of the result in the Scopus database

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