Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F10%3A10077565" target="_blank" >RIV/00216208:11320/10:10077565 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance study

Original language description

We investigated the simultaneous effect of monovalent and divalent cations on hybridization of DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrated that divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium. This trend is opposite to that established for oligonucleotides in a solution. Moreover, the selected saline composition of buffer improved the discrimination of complementary and point mismatched DNA targets.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GA202%2F09%2F0193" target="_blank" >GA202/09/0193: Formation and dynamics of nucleic acid motifs involved in regulation of gene expression</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

38

Issue of the periodical within the volume

20

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

000284165100051

EID of the result in the Scopus database

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