Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00550067" target="_blank" >RIV/61388971:_____/21:00550067 - isvavai.cz</a>

Alternative codes found

RIV/44555601:13440/20:43895720

Result on the web

<a href="https://link.springer.com/article/10.1007/s12223-020-00833-1" target="_blank" >https://link.springer.com/article/10.1007/s12223-020-00833-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-020-00833-1" target="_blank" >10.1007/s12223-020-00833-1</a>

Result language

angličtina

Original language name

Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods

Original language description

The microbial biofilms are ubiquitous in nature and represent important biological entities that affect various aspects of human life. As such, they attracted considerable attention during last decades, with the factors affecting the biofilm development being among the frequently studied topics. In our work, the biofilm was cultivated on the surface of polypropylene fibers in a nutrient medium inoculated by the suspension of two unsterile soils. The effects of ionic strength and valence of salt on the amount of the produced biofilm and on composition of biofilm microbial communities were investigated. The effect of valence was significant in some OTUs: Arthrobacter/Pseudarthrobacter/Paenarthrobacter and Bacillus with positive response to monovalent salt (KCl) and Streptomyces, Lysinibacillus, Pseudomonas, and Ensifer with positive response to divalent salt (MgSO4). The significant preference for a certain concentration of salts was observed in the case of OTUs Agrobacterium, Bacillus (both 100 mM), and Brevundimonas (30 mM). A new quantification method based on measuring of oxidizable organic carbon in biofilm biomass, based on dichromate oxidation, was used. We compared the results obtained using this method with results of crystal violet destaining and measuring of extracted DNA concentration as proxies of the biofilm biomass. The dichromate oxidation is simple, inexpensive, and fast, and our results show that it may be more sensitive than crystal violet destaining. The highest biomass values tended to associate with high concentrations of the divalent salt. This trend was not observed in treatments where the monovalent salt was added. Our data confirm the importance of inorganic ions for biofilm composition and biomass accumulation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA17-09946S" target="_blank" >GA17-09946S: Soil biofilms as collaborative entities: emergence and dynamics in heterogeneous environment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

1874-9356

Volume of the periodical

66

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

133-143

UT code for WoS article

000584027000001

EID of the result in the Scopus database

2-s2.0-85093962596