Aspergillus fumigatus DBM 4057 biofilm formation is inhibited by chitosan, in contrast to baicalein and rhamnolipid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901837" target="_blank" >RIV/60461373:22330/16:43901837 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11274-016-2146-9" target="_blank" >http://dx.doi.org/10.1007/s11274-016-2146-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-016-2146-9" target="_blank" >10.1007/s11274-016-2146-9</a>

Result language

angličtina

Original language name

Aspergillus fumigatus DBM 4057 biofilm formation is inhibited by chitosan, in contrast to baicalein and rhamnolipid

Original language description

The biofilms of filamentous-forming fungi are a novel and still insufficiently understood research topic. We have studied Aspergillus fumigatus, an ubiquitous opportunistic pathogenic fungus, as a representative model for a study of biofilm formation by filamentous fungi and for assessing the potential anti-biofilm activity of natural substances. The activity of antibiotic amphotericin B and selected natural substances: baicalein, chitosan and rhamnolipid was studied. The minimum suspension inhibitory concentrations ( MIC) were determined and the biofilm susceptibility was investigated by determining the metabolic activity of sessile cells ( XTT assay) and total biofilm biomass ( crystal violet staining). Significant time-dependent differences in substances' anti-biofilm activity were observed. Images of A. fumigatus biofilm were obtained by Cellavista automatic light microscope and spinning disc confocal microscopy. Baicalein and rhamnolipid were not found as suitable substances for inhibition of the A. fumigatus biofilm formation, as neither of the substances inhibited the sessile cells metabolic activity or the total biofilm biomass even at tenfold MIC after 48 h. In contrast, chitosan at 10 9 MIC ( 25 mu g mL(-1)), suppressed the biofilm metabolic activity by 90 % and the total biofilm biomass by 80 % even after 72 h of cultivation. Amphotericin B inhibited only 14 % of total biofilm biomass ( crystal violet staining) and 35 % of metabolic activity ( XTT assay) of adherent cells under the same conditions. Our results therefore suggest chitosan as potential alternative for treating A. fumigatus biofilm-associated infections.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

—

Project

<a href="/en/project/GA14-23597S" target="_blank" >GA14-23597S: Impact of environmental factors on biofilm detachment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

World Journal of Microbiology and Biotechnology

ISSN

0959-3993

e-ISSN

—

Volume of the periodical

32

Issue of the periodical within the volume

187

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000385145400014

EID of the result in the Scopus database

—