Detection of microscopic filamentous fungal biofilms – Choosing the suitable methodology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43925329" target="_blank" >RIV/60461373:22330/23:43925329 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0167701223000106" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0167701223000106</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2023.106676" target="_blank" >10.1016/j.mimet.2023.106676</a>
Alternative languages
Result language
angličtina
Original language name
Detection of microscopic filamentous fungal biofilms – Choosing the suitable methodology
Original language description
Microscopic filamentous fungi are ubiquitous microorganisms that adapt very easily to a variety of environmental conditions. Due to this adaptability, they can colonize a number of various surfaces where they are able to start forming biofilms. Life in the form of biofilms provides them with many benefits (increased resistance to desiccation, UV radiation, antimicrobial compounds, and host immune response). The aim of this study is to find a reliable and reproducible methodology to determine biofilm growth of selected microscopic filamentous fungi strains. Several methods (crystal violet staining, MTT assay, XTT assay, resazurin assay) for the determination of total biofilm biomass and its metabolic activity were tested on four fungi – Alternaria alternata, Aspergillus niger, Fusarium culmorum and Fusarium graminearum, and their biofilm was also imaged by spinning disc confocal microscopy using fluorescent dyes. A reproducible biofilm quantification method is essential for the subsequent testing of the biofilm growth suppression using antifungal agents or physical methods. Crystal violet staining was found to be a suitable method for the determination of total biofilm biomass of selected strains, and the MTT assay for the determination of metabolic activity of the biofilms. Calcofluor white and Nile red fluorescent stains successfully dyed the hyphae of microscopic fungi.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiological Methods
ISSN
0167-7012
e-ISSN
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Volume of the periodical
205
Issue of the periodical within the volume
nečíslováno
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
"nečíslováno"
UT code for WoS article
000931532100001
EID of the result in the Scopus database
2-s2.0-85146908602