Drop coating deposition Raman (DCDR) spectroscopy of liposomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F11%3A10081033" target="_blank" >RIV/00216208:11320/11:10081033 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/jrs.2915" target="_blank" >http://dx.doi.org/10.1002/jrs.2915</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jrs.2915" target="_blank" >10.1002/jrs.2915</a>

Result language
angličtina
Original language name
Drop coating deposition Raman (DCDR) spectroscopy of liposomes
Original language description
Drop coating deposition Raman (DCDR) spectroscopy was tested as a potential technique for studying liposomes at very low sample concentrations. We used model liposomes prepared either from 1,2-distearoyl-sn-glycero-3-phospocholine (PC) or from soybean asolectin that is composed of various lipids and thus represents a good model of natural membranes. In both cases, deposited samples formed a dried drop with round shape and a ring of concentrated liposomes at the edge. Spectral mapping showed that maximalRaman intensity originated from the inner part of the edge ring, while Raman signal gradually decreased in both radial directions. Raman spectra exhibited excellent reproducibility of spectral characteristics at different locations in the drop indicating similar conformation and ordering of hydrocarbon lipid chains in the sample. Our results suggest that DCDR spectroscopy can be used for studying lipids in situ, while sensitivity of this technique is at least 2 orders of magnitude highe
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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