Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F13%3A10189236" target="_blank" >RIV/00216208:11320/13:10189236 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.chemphyslip.2013.04.002" target="_blank" >http://dx.doi.org/10.1016/j.chemphyslip.2013.04.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemphyslip.2013.04.002" target="_blank" >10.1016/j.chemphyslip.2013.04.002</a>

Result language

angličtina

Original language name

Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol

Original language description

Drop coating deposition Raman (DCDR) spectroscopy was used to study liposomes (DPPC and asolectin) with growing proportion of cholesterol. Deposited samples of both liposomes on special hydrophobic surface formed a dried drop with a circular shape with aring of concentrated liposomes at the outer edge. The presence of cholesterol in liposome causes a diminishing of the drop size and an increasing in diameter of the ring, but DPPC with 20% of cholesterol forms the compact drop without the ring. Raman spectra contain characteristics of both lipids and cholesterol, liposomes do not change their initial phase state after drying. Spectral mapping shows that maximum Raman intensity originated from the inner part of the ring. Our results suggest that DCDR spectroscopy can be used for studying lipids containing cholesterol in situ.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

<a href="/en/project/GAP208%2F10%2F0376" target="_blank" >GAP208/10/0376: Interaction of antibacterial peptides with the model membranes and possibility of the prediction of their biological activity</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry and Physics of Lipids

ISSN

0009-3084

e-ISSN

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Volume of the periodical

172

Issue of the periodical within the volume

Neuveden

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

1-5

UT code for WoS article

000322415100001

EID of the result in the Scopus database

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