Fluorescence Emission Spectra of Calcofluor Stained Yeast Cell Suspensions: Heuristic Assessment of Basis Spectra for their Linear Unmixing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F12%3A10131292" target="_blank" >RIV/00216208:11320/12:10131292 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10895-012-1058-9" target="_blank" >http://dx.doi.org/10.1007/s10895-012-1058-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10895-012-1058-9" target="_blank" >10.1007/s10895-012-1058-9</a>

Result language
angličtina
Original language name
Fluorescence Emission Spectra of Calcofluor Stained Yeast Cell Suspensions: Heuristic Assessment of Basis Spectra for their Linear Unmixing
Original language description
Fluorescence emission spectra of yeast cell suspensions stained with calcofluor have recently been identified as promising markers of variations in the quality of yeast cell wall. It is shown in this paper how the raw fluorescence spectra of calcofluor can be transformed to reliable spectral signatures of cell wall quality, which are ndependent of actual dye-to-cell concentrations of examined cell suspensions. Moreover, the presented approach makes it possible to assess basis fluorescence spectra that allows for the spectral unmixing of raw fluorescence spectra in terms of respective fluorescence contributions of calcofluor solvated in the suspension medium and bound to yeast cell walls.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—

Project
<a href="/en/project/GAP205%2F10%2F1121" target="_blank" >GAP205/10/1121: Biophysical method of monitoring the activity of multidrug resistance systems and membrane potential changes in yeast cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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