Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F10%3A10082434" target="_blank" >RIV/00216208:11320/10:10082434 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Solvatochromic Effect in the Optical Spectra of Calcofluor and its Relation to Fluorescent Staining of Yeast Cell Walls
Original language description
Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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