Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F12%3A10131297" target="_blank" >RIV/00216208:11320/12:10131297 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10863-012-9458-8" target="_blank" >http://dx.doi.org/10.1007/s10863-012-9458-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10863-012-9458-8" target="_blank" >10.1007/s10863-012-9458-8</a>

Result language
angličtina
Original language name
Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions
Original language description
The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C-3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C-3(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts.Spectral analysis of synchronously scanned diS-C-3(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellu
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/GAP205%2F10%2F1121" target="_blank" >GAP205/10/1121: Biophysical method of monitoring the activity of multidrug resistance systems and membrane potential changes in yeast cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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