Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10288249" target="_blank" >RIV/00216208:11320/14:10288249 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s10895-013-1323-6" target="_blank" >http://dx.doi.org/10.1007/s10895-013-1323-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10895-013-1323-6" target="_blank" >10.1007/s10895-013-1323-6</a>

Result language

angličtina

Original language name

Complementary Methods of Processing diS-C3(3) Fluorescence Spectra Used for Monitoring the Plasma Membrane Potential of Yeast: Their Pros and Cons

Original language description

Carbocyanine dye diS-C-3(3) was repeatedly employed in monitoring the plasma membrane potential of yeast and other living cells. Four methods of measuring and evaluating probe fluorescence signal were used in different studies, based on following fluorescence parameters: fluorescence intensity emitted within a certain spectral interval, F(580)/F(560) fluorescence emission ratio, wavelength of emissionspectrum maximum, and the ratio of respective fluorescence intensities corresponding to the diS-C-3(3) bound to cytosolic macromolecules and remaining dissolved in the aqueous cell medium (i.e., unbound, or free). We have shown that data corresponding to the three latter spectral assessments of diS-C-3(3) accumulation in cells is mutually convertible, which means that their alternative use cannot lead to ambiguities in the interpretation of the results of biological experiments.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Fluorescence

ISSN

1053-0509

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

541-547

UT code for WoS article

000334183100030

EID of the result in the Scopus database

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