Depolarization affects the lateral microdomain structure of yeast plasma membrane

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F15%3A10314842" target="_blank" >RIV/00216208:11320/15:10314842 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/febs.13156" target="_blank" >http://dx.doi.org/10.1111/febs.13156</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/febs.13156" target="_blank" >10.1111/febs.13156</a>

Result language

angličtina

Original language name

Depolarization affects the lateral microdomain structure of yeast plasma membrane

Original language description

We report the transmembrane voltage-induced lateral reorganization of highly-ordered lipid microdomains in the plasma membrane of living Saccharomycescerevisiae. Using trans-parinaric acid (all-trans-9,11,13,15-octadecatetraenoic acid) as a probe of lipid order and different methods of membrane depolarization, we found that depolarization always invokes a significant reduction in the amount of gel-like microdomains in the membrane. Different depolarization mechanisms, including the application of ionophores, cell depolarization by an external electric field, depolarization by proton/hexose co-transport facilitated by HUP1 protein and a reduction of membrane potential caused by compromised respiration efficiency, yielded the same results independently of the yeast strain used. The data suggest that the voltage-induced reorganization of lateral membrane structure could play significant role in fast cellular response to acute stress conditions, as well as in other membrane microdomain-rel

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

<a href="/en/project/GAP205%2F12%2F0720" target="_blank" >GAP205/12/0720: Role of membrane potential in the lateral microdomain organization of the plasma membrane</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Journal

ISSN

1742-464X

e-ISSN

—

Volume of the periodical

282

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

419-434

UT code for WoS article

000348901600001

EID of the result in the Scopus database

2-s2.0-84921803051