Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F16%3A10322380" target="_blank" >RIV/00216208:11320/16:10322380 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/16:10322380
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/mbo3.351/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/mbo3.351/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mbo3.351" target="_blank" >10.1002/mbo3.351</a>

Result language
angličtina
Original language name
Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis
Original language description
Giardia intestinalis is an important single-celled human pathogen. Interestingly, this organism has two equal-sized transcriptionally active nuclei, each considered diploid. By evaluating condensed chromosome numbers and visualizing homologous chromosomes by fluorescent in situ hybridization, we determined that the Giardia cells are constitutively aneuploid. We observed karyotype interand intra-population heterogeneity in eight cell lines from two clinical isolates, suggesting constant karyotype evolution during in vitro cultivation. High levels of chromosomal instability and frequent mitotic missegregations observed in four cell lines correlated with a proliferative disadvantage and growth retardation. Other cell lines, although derived from the same clinical isolate, revealed a stable yet aneuploid karyotype. We suggest that both chromatid missegregations and structural rearrangements contribute to shaping the Giardia genome, leading to whole-chromosome aneuploidy, unequal gene distribution, and a genomic divergence of the two nuclei within one cell. Aneuploidy in Giardia is further propagated without p53-mediated cell cycle arrest and might have been a key mechanism in generating the genetic diversity of this human pathogen.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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