Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10282223" target="_blank" >RIV/00216208:11110/15:10282223 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00412-014-0486-5" target="_blank" >http://dx.doi.org/10.1007/s00412-014-0486-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00412-014-0486-5" target="_blank" >10.1007/s00412-014-0486-5</a>

Result language

angličtina

Original language name

Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Original language description

During mitotic prophase, chromosomes of the pathogenic unicellular eukaryote Giardia intestinalis condense in each of the cell's two nuclei. In this study, Giardia chromosomes were investigated using light microscopy, high-resolution field emission scanning electron microscopy, and in situ hybridization. For the first time, we describe the overall morphology, condensation stages, and mitotic segregation of these chromosomes. Despite the absence of several genes involved in the cohesion and condensationpathways in the Giardia genome, we observed chromatin organization similar to those found in eukaryotes, i.e., 10-nm nucleosomal fibrils, 30-nm fibrils coiled to chromomeres or in parallel arrangements, and closely aligned sister chromatids. DNA molecules of Giardia terminate with telomeric repeats that we visualized on each of the four chromatid endings of metaphase chromosomes. Giardia chromosomes lack primary and secondary constrictions, thus preventing their classification based on t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FN - Epidemiology, infection diseases and clinical immunology

OECD FORD branch

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Project

<a href="/en/project/GAP305%2F12%2F1248" target="_blank" >GAP305/12/1248: Maintenance of chromosome integrity in Giardia intestinalis, a unicellular bi-nucleated eukaryote with minimalistic genome</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromosoma

ISSN

0009-5915

e-ISSN

—

Volume of the periodical

124

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

14

Pages from-to

81-94

UT code for WoS article

000350304400007

EID of the result in the Scopus database

2-s2.0-84923699742