Quantification of Polyphosphate in Microalgae by Raman Microscopy and by a Reference Enzymatic Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F17%3A10368587" target="_blank" >RIV/00216208:11320/17:10368587 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acs.analchem.7b02393" target="_blank" >http://dx.doi.org/10.1021/acs.analchem.7b02393</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.7b02393" target="_blank" >10.1021/acs.analchem.7b02393</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of Polyphosphate in Microalgae by Raman Microscopy and by a Reference Enzymatic Assay
Original language description
Polyphosphates have occurred in living cells early in evolution and microalgae contain these important polymers in their cells. Progress in research of polyphosphate metabolism of these ecologically as well as biotechnologically important microorganisms is hampered by the lack of rapid quantification methods. Experiments with the green alga Chlorella vulgaris presented here compared polyphosphate extraction in water, methanol-chloroform, and phenol-chloroform followed by polyphosphate purification by binding to silica columns or ethanol precipitation. The phenol-chloroform extraction of C.vulgaris followed by ethanol precipitation of polyphosphate was shown to be superior to the other tested method variants. Recovery test of added polyphosphate standard to algal biomass showed that the method is accurate. Using this biochemical assay as a validated reference, we show that 2-dimensional, confocal Raman microscopy can serve as a linear proxy for polyphosphate in C. vulgaris with R-2 up to 0.956. With this, polyphosphate quantification can be shortened by use of Raman microscopy from days to hours and, additionally, information about intracellular distribution of polyphosphate and heterogeneity among individual cells in algal culture can be obtained. This offers new insights into the dynamics and role of these polymers crucial for phosphorus uptake and storage. This analytical capability is of particular practical importance because algae aid phosphorus sequestration from wastewater and the thus enriched biomass may serve as organic fertilizer. Both these applications have a strong potential in a future sustainable, circular bioeconomy.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10301 - Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect)
Result continuities
Project
<a href="/en/project/GA17-06264S" target="_blank" >GA17-06264S: Growth and division in stable isotopes – beyond metabolic labeling</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
89
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
12006-12013
UT code for WoS article
000416498100019
EID of the result in the Scopus database
2-s2.0-85035034239