RNA-directed off/on switch of RNase H activity using boronic ester formation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F17%3A10370893" target="_blank" >RIV/00216208:11320/17:10370893 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1039/c7ob02145c" target="_blank" >http://dx.doi.org/10.1039/c7ob02145c</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c7ob02145c" target="_blank" >10.1039/c7ob02145c</a>

Result language
angličtina
Original language name
RNA-directed off/on switch of RNase H activity using boronic ester formation
Original language description
RNase H is a non-specific endonuclease which degrades selectively the RNA strand in DNA/RNA duplexes. We demonstrate in the present study that 5'-boronic acid modified oligonucleotides hybridized to a RNA target sequence converts RNase H to an inactivated enzyme complex. The dynamic formation of a boronate ester upon addition of a diol moiety disrupts the enzyme-inhibitor complex and reactivates RNase H. Moreover, we show that reactivation of RNase H function can also be engineered through short RNA trimers inputs that fashion RNase H from a non-specific DNA-guided enzyme into an informational and programmable RNA-guided one. Examples of programmable RNA recognition and cleavage illustrate the potential of this new stimuli-responsive system.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GA15-06785S" target="_blank" >GA15-06785S: Nucleic Acid Regulatory Sequences - polymorphism, dynamics, and interactions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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