The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00524333" target="_blank" >RIV/61388971:_____/20:00524333 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10414337 RIV/00216208:11320/20:10414337
Result on the web
<a href="https://www.embopress.org/doi/abs/10.15252/embj.2019102500" target="_blank" >https://www.embopress.org/doi/abs/10.15252/embj.2019102500</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embj.2019102500" target="_blank" >10.15252/embj.2019102500</a>
Alternative languages
Result language
angličtina
Original language name
The torpedo effect in Bacillus subtilis: RNase J1 resolves stalled transcription complexes
Original language description
RNase J1 is the major 5 '-to-3 ' bacterial exoribonuclease. We demonstrate that in its absence, RNA polymerases (RNAPs) are redistributed on DNA, with increased RNAP occupancy on some genes without a parallel increase in transcriptional output. This suggests that some of these RNAPs represent stalled, non-transcribing complexes. We show that RNase J1 is able to resolve these stalled RNAP complexes by a ´torpedo´ mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with RNAP. A heterologous enzyme, yeast Xrn1 (5 '-to-3 ' exonuclease), is less efficient than RNase J1 in resolving stalled Bacillus subtilis RNAP, suggesting that the effect is RNase-specific. Our results thus reveal a novel general principle, whereby an RNase can participate in genome-wide surveillance of stalled RNAP complexes, preventing potentially deleterious transcription-replication collisions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EMBO Journal
ISSN
0261-4189
e-ISSN
—
Volume of the periodical
39
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
e102500
UT code for WoS article
000502667400001
EID of the result in the Scopus database
2-s2.0-85076793026