Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F21%3A00549270" target="_blank" >RIV/61388971:_____/21:00549270 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/nar/article/49/18/10221/6374178" target="_blank" >https://academic.oup.com/nar/article/49/18/10221/6374178</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/nar/gkab803" target="_blank" >10.1093/nar/gkab803</a>

Result language
angličtina
Original language name
Critical Reviews and Perspectives beta-CASP proteins removing RNA polymerase from DNA: when a torpedo is needed to shoot a sitting duck
Original language description
During the first step of gene expression, RNA polymerase (RNAP) engages DNA to transcribe RNA, forming highly stable complexes. These complexes need to be dissociated at the end of transcription units or when RNAP stalls during elongation and becomes an obstacle ('sitting duck') to further transcription or replication. In this review, we first outline the mechanisms involved in these processes. Then, we explore in detail the torpedo mechanism whereby a 5'-3' RNA exonuclease (torpedo) latches itself onto the 5' end of RNA protruding from RNAP, degrades it and upon contact with RNAP, induces dissociation of the complex. This mechanism, originally described in Eukaryotes and executed by Xrn-type 5'-3' exonucleases, was recently found in Bacteria and Archaea, mediated by beta-CASP family exonucleases. We discuss the mechanistic aspects of this process across the three kingdoms of life and conclude that 5'-3' exoribonucleases (beta-CASP and Xrn families) involved in the ancient torpedo mechanism have emerged at least twice during evolution.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA19-12956S" target="_blank" >GA19-12956S: Pivotal aspects of mycobacterial transcription: The promoter-recognizing subunit of RNAP, SigA, and its interactions with a newly identified factor.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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