Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00538228" target="_blank" >RIV/61388971:_____/20:00538228 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/20:00538228 RIV/68378050:_____/20:00538228 RIV/00216224:14740/20:00118335 RIV/00216208:11320/20:10423322
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749160/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749160/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-020-20158-4" target="_blank" >10.1038/s41467-020-20158-4</a>
Alternative languages
Result language
angličtina
Original language name
Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase
Original language description
RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors for recovery from stalled states and adaptation to environmental changes. Here, we investigated the mechanism by which a helicase-like factor HelD recycles RNAP. We report a cryo-EM structure of a complex between the Mycobacterium smegmatis RNAP and HelD. The crescent-shaped HelD simultaneously penetrates deep into two RNAP channels that are responsible for nucleic acids binding and substrate delivery to the active site, thereby locking RNAP in an inactive state. We show that HelD prevents non-specific interactions between RNAP and DNA and dissociates stalled transcription elongation complexes. The liberated RNAP can either stay dormant, sequestered by HelD, or upon HelD release, restart transcription. Our results provide insights into the architecture and regulation of the highly medically-relevant mycobacterial transcription machinery and define HelD as a clearing factor that releases RNAP from nonfunctional complexes with nucleic acids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
—
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
6419
UT code for WoS article
000608512400003
EID of the result in the Scopus database
2-s2.0-85097759044