Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00600304" target="_blank" >RIV/61388971:_____/24:00600304 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/24:00600304 RIV/61388963:_____/24:00600304 RIV/00216224:90242/24:00139154 RIV/00216208:11310/24:10487061
Result on the web
<a href="https://www.nature.com/articles/s41467-024-52891-5" target="_blank" >https://www.nature.com/articles/s41467-024-52891-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-024-52891-5" target="_blank" >10.1038/s41467-024-52891-5</a>
Alternative languages
Result language
angličtina
Original language name
Mycobacterial HelD connects RNA polymerase recycling with transcription initiation
Original language description
Mycobacterial HelD is a transcription factor that recycles stalled RNAP by dissociating it from nucleic acids and, if present, from the antibiotic rifampicin. The rescued RNAP, however, must disengage from HelD to participate in subsequent rounds of transcription. The mechanism of release is unknown. We show that HelD from Mycobacterium smegmatis forms a complex with RNAP associated with the primary sigma factor sigma A and transcription factor RbpA but not CarD. We solve several structures of RNAP-sigma A-RbpA-HelD without and with promoter DNA. These snapshots capture HelD during transcription initiation, describing mechanistic aspects of HelD release from RNAP and its protective effect against rifampicin. Biochemical evidence supports these findings, defines the role of ATP binding and hydrolysis by HelD in the process, and confirms the rifampicin-protective effect of HelD. Collectively, these results show that when HelD is present during transcription initiation, the process is protected from rifampicin until the last possible moment.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Communications
ISSN
2041-1723
e-ISSN
2041-1723
Volume of the periodical
15
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
8740
UT code for WoS article
001335963600016
EID of the result in the Scopus database
2-s2.0-85205949448