Erg6 gene is essential for stress adaptation in Kluyveromyces lactis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F18%3A10386902" target="_blank" >RIV/00216208:11320/18:10386902 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1093/femsle/fny265" target="_blank" >https://doi.org/10.1093/femsle/fny265</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fny265" target="_blank" >10.1093/femsle/fny265</a>
Alternative languages
Result language
angličtina
Original language name
Erg6 gene is essential for stress adaptation in Kluyveromyces lactis
Original language description
We investigated the effect of Kluyveromyces lactis ERG6 gene deletion on plasma membrane function and showed increased susceptibility of mutant cells to salt stress, cationic drugs and weak organic acids. Contrary to Saccharomyces cerevisiae, Klerg6 mutant cells exhibited increased tolerance to tunicamycin. The content of cell wall polysacharides did not significantly vary between wild-type and mutant cells. Although the expression of the NAD+-dependent glycerol 3-phosphate dehydrogenase (KlGPD1) in the Klerg6 mutant cells was only half of that in the parental strain, it was induced in the presence of calcofluor white. Also, cells exposed to this drug accumulated glycerol. The absence of KlErg6p led to plasma membrane hyperpolarization but had no statistically significant influence on the plasma membrane fluidity. We propose that the phenotype of Klerg6 mutant cells to a large extent was a result of the reduced activity of specific plasma membrane proteins that require proper lipid composition for full activity.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Microbiology Letters
ISSN
0378-1097
e-ISSN
—
Volume of the periodical
365
Issue of the periodical within the volume
23
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
1-8
UT code for WoS article
000452557600009
EID of the result in the Scopus database
2-s2.0-85057206146