Distinct leukocyte populations and cytokine secretion profiles define tumoral and peritumoral areas in renal cell carcinoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11510%2F24%3A10475558" target="_blank" >RIV/00216208:11510/24:10475558 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/24:10475558 RIV/00216208:11130/24:10475558 RIV/00064203:_____/24:10475558 RIV/00064165:_____/24:10475558
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bXh2DvJQHa" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=bXh2DvJQHa</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tranon.2024.101891" target="_blank" >10.1016/j.tranon.2024.101891</a>
Alternative languages
Result language
angličtina
Original language name
Distinct leukocyte populations and cytokine secretion profiles define tumoral and peritumoral areas in renal cell carcinoma
Original language description
Renal cell carcinoma (RCC) is a common malignancy frequently diagnosed at the metastatic stage. We performed a comprehensive analysis of the tumor immune microenvironment (TIME) in RCC patients, including the peritumoral tissue microenvironment, to characterize the phenotypic patterns and functional characteristics of infiltrating immune cells. T cells from various compartments (peripheral blood, tumor, peritumoral area, and adjacent healthy renal tissue) were assessed using flow cytometry and Luminex analyses, both before and after T cell-specific stimulation, to evaluate activation status and migratory potential. Our findings demonstrated that tumor-infiltrating lymphocytes (TILs) exhibited heightened cytokine production compared to peritumoral T cells (pTILs), acting as the primary source of cytotoxic markers (IFN-γ, granzyme B, and FasL). CD8(+) T cells primarily employed Fas Ligand for cytotoxicity, while CD4(+) T cells relied on CD107a. In addition, a statistically significant negative correlation between patient mortality and the presence of CD4(+)CD107(+) pTILs was demonstrated. The engagement with the PD-1/PD-L1 pathway was also more evident in CD4(+) and CD8(+) pTILs as opposed to TILs. PD-L1 expression in the non-leukocyte fraction of the tumor tissue was relatively lower than in their leukocytic counterparts and upon stimulation, peripheral blood T cells displayed much stronger responses to stimulation than TILs and pTILs. Our results suggest that tumor and peritumoral T cells exhibit limited responsiveness to additional activation signals, while peripheral T cells retain their capacity to respond to stimulatory signals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Translational Oncology
ISSN
1936-5233
e-ISSN
—
Volume of the periodical
42
Issue of the periodical within the volume
April
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
101891
UT code for WoS article
001180356900001
EID of the result in the Scopus database
2-s2.0-85183991440