Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00002251" target="_blank" >RIV/00216224:14110/00:00002251 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Schwann cells migrating in absence of the axons in the basal lamina tubes express immunoreactivity for laminins, fibronectin, thrombospondin, and chondroitin sulfate

Original language description

The acellular nerve segment is thought to be a method of choice for reparation of nerve defect. Repeated periods of freezing and thawing are frequently used for preparation of standard acellular nerve segment in experimental application. It was believedthat the basal lamina tubes and endoneurial extracellular matrix molecules survive the procedures and promote the growth of regenerating axons. We used the model of freeze-treated segment of the distal nerve stump to study a molecular content of the endoneurium in absence and presence of Schwann cells. The nerve segment, marked out at the end of distal stump of transected rat sciatic nerve, was repeatedly frozen and thawed (5 times) to evacuate all resident cells. Serial longitudinal frozen sections, 10microm thick, were incubated with monoclonal antibodies against laminin-1, -2, and -3, fibronectin, thrombospondin, as well as chondroitin sulfate. The position of Schwann cells was detected in subsequent sections or simultaneously by im

Czech name

Migrující Schwannovy buňky při absenci axonů vykazují reakci na laminin, fibronectin, thrombospondin a chondroitin sulfat

Czech description

Migrující Schwanovy buňky bez přítomnosti axonů vykazují imunohistochemickou reakci na laminin, fbronectin, thrombospondin a chondroitin sulfát.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

<a href="/en/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Cellular and extracellular environment for the axon regeneration following injury of the spinal roots and peripheral nerves</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Eur. J. Neurosci.

ISSN

0953-816X

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

Suppl. 11

Country of publishing house

GB - UNITED KINGDOM

Number of pages

1

Pages from-to

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UT code for WoS article

000088236601655

EID of the result in the Scopus database

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