Stability of yeast cytoskeleton under cell stress

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00003450" target="_blank" >RIV/00216224:14110/00:00003450 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Stability of yeast cytoskeleton under cell stress
Original language description
The effects of heat, osmotic and radiation stresses on cells exposed to a lethal heat shock were studied on the model yeast Saccharomyces cerevisiae CCY 21-4-59. Cell thermoresistance or thermosensitivity was detected by their ability to form colonies (CFU) at 25 degrees C. After the transfer from 25 to 46 degrees C most cells lost their viability after a 10-min incubation and this temperature was then referred to as lethal heat shock. A 30-min preincubation of cells at 37 or 41 degrees C (referred to here as a mild heat stress) led to an increase of their resistance to the lethal heat shock. Western blotting technique (+ ECL) revealed that during mild heat stress the concentration of Hsp 104 in cells increased, while in controls only traces of this protein were detected. The Hsp 104 synthesis was apparently adaptive, because cycloheximide present during the mild heat stress effectively prevented the increase of cell thermoresistance. Accumulation of Hsp 104 was detected also by indire
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GA204%2F00%2F0394" target="_blank" >GA204/00/0394: The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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