A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00005108" target="_blank" >RIV/00216224:14110/00:00005108 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures
Original language description
The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heatshock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C forup to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
—

Project
<a href="/en/project/GA204%2F00%2F0394" target="_blank" >GA204/00/0394: The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)