A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F02%3A00005820" target="_blank" >RIV/00216224:14110/02:00005820 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A QUANTITATIVE IMMUNOFLUORESCENCE ANALYSIS OF SOME ECM MOLECULES AND NEUROTROPHINS ALONGSIDE AFFERENT AND MOTOR AXONS IN THE DORSAL AND VENTRAL SPINAL ROOTS

Original language description

The dorsal and ventral spinal roots contain a dominant amount of afferent and motor axons, respectively. Besides differences of intrinsic capacities of the motor and primary sensory neurons for axon regeneration, we hypothesize different cellular and extracellular conditions in the dorsal and ventral roots based on various types of axons. Chondroitin sulfate proteoglycan (CSPG), fibronectin (FN), tenascin (TN) and thrombospondin (TSP) as well as BDNF, NGF and NT-3 were investigated by optimized indirectimmunofluorescence method. Intensity of immunofluorescence staining was compared using the computer-assisted image analysis. The measurement of immunofluorescence intensity revealed that the endoneurial ECM of the dorsal roots was immunostained for thestudied molecules at a higher intensity than in the ventral roots. Two weeks after root transection, the immunofluorescence intensity for ECM molecules decreased in comparison to intact roots. In addition, a higher intensity for CSPG rema

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

<a href="/en/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Cellular and extracellular environment for the axon regeneration following injury of the spinal roots and peripheral nerves</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Glia

ISSN

0894-1491

e-ISSN

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Volume of the periodical

Suppl. 1

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

"S42"

UT code for WoS article

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EID of the result in the Scopus database

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