Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F02%3A00006843" target="_blank" >RIV/00216224:14110/02:00006843 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a Lucia software

Original language description

The endoneurial ECM is produced by Schwann cells and fibroblasts under the control of the axons. The dorsal and ventral spinal roots contain different types of axons, but no information is available about differences of their ECM content. The present paper demonstrates a comparison of immunofluorescence intensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of rat dorsal and ventral spinal roots. Sections through the dorsal and ventral roots wereincubated simultaneously for indirect immunofluorescence detection of studied epitopes. The brightness of immunofluorescence staining was assessed by computer-assisted image analysis (Lucia G, Laboratory Imaging Ltd., Prague) using interactive segmentation of digitized pictures to select the areas of measurement. The results revealed quantitative differences of the endoneurial ECM content of the spinal dorsal and ventral roots probably corresponding to the presence of different types of

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

<a href="/en/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Cellular and extracellular environment for the axon regeneration following injury of the spinal roots and peripheral nerves</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta histochemica

ISSN

0065-1281

e-ISSN

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Volume of the periodical

104

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

371

UT code for WoS article

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EID of the result in the Scopus database

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